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Literature summary for 5.4.2.6 extracted from
- Discovery of a kojibiose phosphorylase in Escherichia coli K-12 (2018), Biochemistry, 57, 2857-2867 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose 1-phosphate | substrate inhibition is observed at concentrations of beta-D-glucose-1-phosphate greater than 0.2 mM | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 0.09 | - |
beta-D-mannose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 0.47 | - |
beta-D-galactose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 1.19 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 10.5 | - |
beta-D-allose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation constant is 0.135 mM. In the absence of added Mg2+, the observed catalytic activity is less than 2% of the turnover observed in the presence of 2.0 mM Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-Glucose 1-phosphate | Escherichia coli | - |
beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | Escherichia coli MG1655 | - |
beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-glucose 6-phosphate | Escherichia coli | - |
beta-D-glucose 1-phosphate | - |
r | |
| beta-D-glucose 6-phosphate | Escherichia coli MG1655 | - |
beta-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77366 | - |
- |
| Escherichia coli MG1655 | P77366 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-allose-1-phosphate | - |
Escherichia coli | beta-D-allose-6-phosphate | - |
r | |
| beta-D-allose-1-phosphate | - |
Escherichia coli MG1655 | beta-D-allose-6-phosphate | - |
r | |
| beta-D-allose-6-phosphate | - |
Escherichia coli | beta-D-allose-1-phosphate | - |
r | |
| beta-D-allose-6-phosphate | - |
Escherichia coli MG1655 | beta-D-allose-1-phosphate | - |
r | |
| beta-D-galactose-1-phosphate | - |
Escherichia coli | beta-D-galactose-6-phosphate | - |
r | |
| beta-D-galactose-6-phosphate | - |
Escherichia coli | beta-D-galactose-1-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | - |
Escherichia coli | beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | the enzyme exhibits less than 1% of the catalytic activity (relative to beta-D-glucose-1-phosphate) with alpha-D-glucose-1-phosphate | Escherichia coli | beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | - |
Escherichia coli MG1655 | beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | the enzyme exhibits less than 1% of the catalytic activity (relative to beta-D-glucose-1-phosphate) with alpha-D-glucose-1-phosphate | Escherichia coli MG1655 | beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-glucose 6-phosphate | - |
Escherichia coli | beta-D-glucose 1-phosphate | - |
r | |
| beta-D-glucose 6-phosphate | - |
Escherichia coli MG1655 | beta-D-glucose 1-phosphate | - |
r | |
| beta-D-mannose-1-phosphate | - |
Escherichia coli | beta-D-mannose-6-phosphate | - |
r | |
| beta-D-mannose-6-phosphate | - |
Escherichia coli | beta-D-mannose-1-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YcjU | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 21 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 116 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
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